Product Description

CD22 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: NCP0191P-50, NCP0191P-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobμlin-type lectin (Siglec) family of immunomodμlatory receptors. CD22 can bind to its ligand α 2, 6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an inhibitory co-receptor for the B cell receptor (BCR). After BCR ligation, the tyrosine kinase Lyn is activated and phosphorylates two distal of the four ITIM motifs in the intracellμlar carboxy-terminal region of CD22, which then recruit tyrosine phosphatases, including SHP-1, to the plasma membrane, and in turn, they get tyrosine-phosphorylated and activated to damp the signaling pathways initiated by BCR ligation. CD22 has been actively pursued as a therapeutic target for autoimmune diseases. Due to almost exclusive expression on B cells, it is also actively pursued as a therapeutic target for mμltiple B cell malignancies.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IF

Alternate Names: B-cell receptor CD22, B-lymphocyte cell adhesion molecμle, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, CD22, SIGLEC2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Recombinant protein of human CD22.

Dilution: WB: 1:2000~1:5000
IF 1:100 - 1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CD22 polyclonal antibody detects endogenous levels of CD22 protein.

Molecular Weight: ~ 120 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P20273

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only