CD25 Rat Monoclonal Antibody-MB65832

CD25 Rat Monoclonal Antibody

Catalogue Numbers:MB65832-50, MB65832-100

Product: Rat IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P01590

Host: Rat

Reactivity: Mouse

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation. IL-2 also promotes peripheral development of regulatory T cells (Tregs). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells. The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc. IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ. γc is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21. Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways.

Purification and Purity: The monoclonal antibody was affinity-purified from rat antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bioworld Molecular Weight: ~ 30 kDa

Specificity: Recognizes mouse CD25

Note: For research use only, not for use in diagnostic procedure.
Alternative Name: Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25

Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2

Conjugate: Unconjugated

Modification: Unmodified