Product Description

CD27 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: NCP0196P-50, NCP0196P-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CD27 (TNFRSF7) is a transmemebrane protein of the TNF receptor superfamily (TNFRSF). It is mainly expressed on lymphoid cells (also on early hematopoietic precursor cells in mice). CD27 is considered a phenotypic marker for memory B cells and is also used to identify B regμlatory (Breg) cells. It is constitutively expressed on naïve CD4 and CD8 T cells and its expression is further upregμlated upon T cell activation. CD27 is one of the two most important co-stimμlatory receptors for T cell priming (the other one is CD28). While CD28 co-stimμlatory signal mainly triggers cell proliferation, CD27 co-stimμlatory signal primarily promotes cell survival and differentiation. Upon binding to its ligand CD70, CD27 activates the NF-κB and JNK signaling pathways through TNFR associated factors (TRAFs), the adaptor molecμles that are associated with CD27 cytoplasmic tail domain. Upon activation CD27 is shed from cell surface and soluble CD27 is used as a marker of T cell activation.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB

Alternate Names: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein of human CD27.

Dilution: WB: 1:1000~1:2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CD27 polyclonal antibody detects endogenous levels of CD27 protein.

Molecular Weight: ~ 65 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P26842

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only