CD273 Monoclonal Antibody-MB67189

CD273 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB67189-50, MB67189-100

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9BQ51

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB (1/1000 - 1/4000), IHC (1/50 - 1/200)

Background: Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD273 protein.

Bioworld Molecular Weight: ~ 55 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD273 expression in Nontransfected 293T (A), Transfected PDL2transfected 293T (B) whole cell lysates., Immunocytochemistry analysis of CD273 staining in PD-L2-transfected 293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.

Alternative Name: B7DC; CD273; PDCD1L2; PDL2; Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; CD273

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD273. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified