CD276/B7-H3 Polyclonal Antibody-BS77160

CD276/B7-H3 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS77160-50, BS77160-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: Q5ZPR3 Host: Rabbit Reactivity: Human Applications: WB, IHC All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 Background: The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Bioworld Molecular Weight: 120kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of extracts of U-87MG cells, using CD276/B7-H3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per Lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s., Immunohistochemistry of paraffin-embedded Human tonsil using CD276/B7-H3 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Human liver cancer using CD276/B7-H3 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol. Alternative Name: CD276; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2 Immunogen: A synthetic peptide of human CD276/B7-H3(NP_001019907.1). Conjugate: Unconjugated Modification: Unmodified