Product Description

CD28 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65740-50, BS65740-100

Swiss-Prot: P31041

Host: Rabbit

CD28 Polyclonal Antibody-BS65740

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,

Applications: WB,

All Applications: WB=1:500-2000

Background: Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95% of CD4+ cells and 50% of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.

Purification and Purity: affinity purified by Protein A

Specificity: CD28 Polyclonal Antibody detects endogenous levels of CD28 protein.

Bioworld Molecular Weight: 50 kD，44kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Primary: Anti-CD28 at 1/1000 dilution, Primary: Anti-CD28 at 1/1000 dilution

Alternative Name: CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44.

Immunogen: KLH conjugated synthetic peptide derived from mouse CD28:1-120/218

Modification: Unmodified

Conjugate: Unconjugated