CD294 Polyclonal Antibody -BS67174

CD294 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67174-50, BS67174-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9Y5Y4

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC

Background: Receptor for prostaglandin D2 (PGD2). Coupled to the g (i)-protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca2+mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 135% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of CD294 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD294 expression in HEK293T (A), HuT78 (B), rat spleen (C) whole cell lysates., Immunohistochemical analysis of CD294 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 43 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CRTH2; DL1R; GPR44; Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CD294

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD294. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified