Product Description

CD3 Rat Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65827-50, MB65827-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellμlar signal transduction machinery. This activation process depends mainly on CD3 (Cluster of Differentiation 3), a mμltiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecμles such as ZAP-70 and the p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck.

Category: Primary Antibody

Reactivity: Mouse

Host: Rat

Applications: IF, FC

Alternate Names: T3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, T3G, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain, CD3g

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: γδTCR-positive T-T hybridoma D1

Dilution: IF (1/50 - 1/200)

Purification: The monoclonal antibody was affinity-purified from rat antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes mouse CD3

Molecular Weight: ~ 21 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P22646, P11942

Product: Rat IgG2b kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Research Use Only