Product Description

CD300A Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0387-500, NCP0387-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CD300a is an inhibitory receptor in the Ig superfamily, a type I transmembrane protein containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) on its cytoplasmic tail. Human CD300a (CMRF-35H, IRp60) and mouse CD300a (CLM-8, LMIR-1, MAIR-I) are functional orthologs, expressed by monocytes, granμlocytes, mast cells, and subsets of B cells. Human, but not mouse, CD300a is also expressed by unstimμlated NK cells and subsets of T cells. Phosphorylated ITIMs are able to recruit different phosphatases, such as SHP-1 and SHP-2, leading to inhibition of cell activation.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~23kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: Q9UGN4

Tag: His-tag

Amino Acid Sequence: LSKCRTVAGPVGGSLSVQCPYEKEHRTLNKYWCRPPQIFLCDKIVETKGSAGKRNGRVSIRDSPANLSFTVTLENLTEEDAGTYWCGVDTPWLRDFHDPVVEVEVSVFPASTSMTPASITAAKTSTITTAFPPVSSTTLFAVGATHSASIQEETEEVVNSQLP

Expression Vector: pet-22b(+)

Research Use Only