CD31 Monoclonal Antibody-MB66677

CD31 Monoclonal Antibody

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Catalogue Numbers: MB66677-50, MB66677-100

Sizes: 50µl, 100µl

Swiss-Prot: P16284

Host: Mouse

Reactivity: Human

Applications: WB, IHC

Background: CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules.

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of CD31 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD31 expression in THP1 (A) whole cell lysates., Immunohistochemical analysis of CD31 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.144). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 135 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31

Immunogen: Purified recombinant human CD31 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified