Product Description

CD31/PECAM-1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6897-50, BS6897-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P16284

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB 1:500 - 1:2000

Background: CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells and endothelial cells, and modulates cell adhesion, endothelial cell migration and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical stress or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain containing binding partners such as PI3-kinase, SHIP, PLCγ and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD31/PECAM-1 polyclonal antibody detects endogenous levels of CD31 protein.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot analysis of extracts of Jurkat cell line, using CD31 antibody.

Alternative Name: Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD31; PECAM1

Immunogen: Recombinant full length Human CD31.

Conjugate: Unconjugated

Modification: Unmodified