Product Description

CD31 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0199-500, NCP0199-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CD31 (Platelet Endothelial Cell Adhesion Molecμle-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecμles, is expressed by circμlating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modμlates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimμli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecμles.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~63kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P16284

Tag: His-tag

Amino Acid Sequence: CAGGAAAATAGCTTCACCATTAATAGTGTTGATATGAAAAGCCTGCCGGATTGGACCGTTCAGAATGGTAAAAATCTGACCCTGCAGTGCTTCGCAGATGTGAGTACCACCAGTCATGTGAAACCGCAGCATCAGATGCTGTTCTATAAAGATGATGTTCTGTTCTATAACATCAGTAGTATGAAAAGTACCGAAAGCTACTTCATTCCGGAAGTGCGTATCTATGATAGTGGTACCTATAAATGCACCGTGATTGTTAATAATAAGGAAAAAACCACCGCCGAATATCAGGTGCTGGTGGAAGGTGTGCCGAGCCCGCGCGTTACCCTGGATAAAAAAGAAGCAATTCAGGGTGGTATTGTTCGCGTGAATTGCAGCGTGCCGGAAGAAAAAGCACCGATTCACTTCACCATTGAAAAACTGGAACTGAATGAAAAAATGGTTAAGCTGAAACGCGAAAAAAATAGTCGCGATCAGAACTTCGTTATTCTGGAATTCCCGGTGGAAGAACAGGATCGTGTTCTGAGCTTCCGCTGTCAGGCCCGCATTATTAGTGGCATTCACATGCAGACCAGCGAAAGCACCAAAAGTGAACTGGTTACCGTGACCGAAAGCTTCAGTACCCCGAAATTCCATATTAGTCCGACCGGCATGATTATGGAAGGCGCACAGCTGCATATTAAATGTACCATTCAGGTTACCCATCTGGCCCAGGAATTCCCGGAAATTATTATTCAGAAAGATAAGGCCATCGTTGCACATAATCGTCATGGTAATAAAGCCGTGTATAGTGTTATGGCCATGGTTGAACATAGTGGTAATTATACCTGTAAAGTGGAAAGTAGCCGTATTAGCAAAGTTAGTAGTATTGTTGTTAACATCACCGAACTGTTCAGTAAACCGGAACTGGAAAGTAGCTTCACCCATCTGGATCAGGGCGAACGTCTGAATCTGAGTTGCAGTATTCCGGGTGCACCGCCGGCCAACTTCACCATTCAGAAAGAAGATACCATTGTGAGTCAGACCCAGGACTTCACCAAAATTGCAAGTAAAAGCGATAGTGGCACCTATATCTGTACCGCCGGCATTGATAAAGTTGTTAAAAAAAGCAACACCGTTCAGATTGTTGTGTGTGAAATGCTGAGTCAGCCGCGCATTAGCTATGATGCACAGTTCGAAGTTATTAAAGGCCAGACCATTGAAGTTCGCTGTGAAAGTATTAGCGGCACCCTGCCGATTAGTTATCAGCTGCTGAAAACCAGTAAAGTGCTGGAAAATAGCACCAAAAATAGCAATGATCCGGCAGTGTTCAAAGATAATCCGACCGAAGATGTGGAATATCAGTGCGTTGCAGATAATTGTCATAGCCATGCCAAAATGCTGAGCGAAGTTCTGCGTGTTAAAGTGATTGCACCGGTGGATGAAGTGCAGATTAGCATTCTGAGCAGCAAAGTGGTGGAAAGCGGTGAAGATATTGTGCTGCAGTGTGCCGTTAATGAAGGCAGTGGTCCGATTACCTATAAATTCTATCGTGAAAAAGAGGGTAAGCCGTTCTATCAGATGACCAGTAATGCCACCCAGGCCTTCTGGACCAAACAGAAAGCAAGTAAAGAACAGGAAGGTGAATATTATTGTACCGCCTTCAATCGCGCCAATCATGCAAGCAGTGTTCCGCGTAGTAAAATTCTGACCGTTCGTGTGATTCTGGCCCCGTGGAAAAAA

Expression Vector: pet-22b(+)

Research Use Only