Product Description

CD31 Recombinant Protein

Catalogue Number: NCP0199-500, NCP0199-1000

Sizes: 500ug, 1mg

Swiss-Prot: P16284

Host: E.coli

Tag: His-tag

AA Sequence: CAGGAAAATAGCTTCACCATTAATAGTGTTGATATGAAAAGCCTGCCGGATTGGACCGTTCAGAATGGTAAAAATCTGACCCTGCAGTGCTTCGCAGATGTGAGTACCACCAGTCATGTGAAACCGCAGCATCAGATGCTGTTCTATAAAGATGATGTTCTGTTCTATAACATCAGTAGTATGAAAAGTACCGAAAGCTACTTCATTCCGGAAGTGCGTATCTATGATAGTGGTACCTATAAATGCACCGTGATTGTTAATAATAAGGAAAAAACCACCGCCGAATATCAGGTGCTGGTGGAAGGTGTGCCGAGCCCGCGCGTTACCCTGGATAAAAAAGAAGCAATTCAGGGTGGTATTGTTCGCGTGAATTGCAGCGTGCCGGAAGAAAAAGCACCGATTCACTTCACCATTGAAAAACTGGAACTGAATGAAAAAATGGTTAAGCTGAAACGCGAAAAAAATAGTCGCGATCAGAACTTCGTTATTCTGGAATTCCCGGTGGAAGAACAGGATCGTGTTCTGAGCTTCCGCTGTCAGGCCCGCATTATTAGTGGCATTCACATGCAGACCAGCGAAAGCACCAAAAGTGAACTGGTTACCGTGACCGAAAGCTTCAGTACCCCGAAATTCCATATTAGTCCGACCGGCATGATTATGGAAGGCGCACAGCTGCATATTAAATGTACCATTCAGGTTACCCATCTGGCCCAGGAATTCCCGGAAATTATTATTCAGAAAGATAAGGCCATCGTTGCACATAATCGTCATGGTAATAAAGCCGTGTATAGTGTTATGGCCATGGTTGAACATAGTGGTAATTATACCTGTAAAGTGGAAAGTAGCCGTATTAGCAAAGTTAGTAGTATTGTTGTTAACATCACCGAACTGTTCAGTAAACCGGAACTGGAAAGTAGCTTCACCCATCTGGATCAGGGCGAACGTCTGAATCTGAGTTGCAGTATTCCGGGTGCACCGCCGGCCAACTTCACCATTCAGAAAGAAGATACCATTGTGAGTCAGACCCAGGACTTCACCAAAATTGCAAGTAAAAGCGATAGTGGCACCTATATCTGTACCGCCGGCATTGATAAAGTTGTTAAAAAAAGCAACACCGTTCAGATTGTTGTGTGTGAAATGCTGAGTCAGCCGCGCATTAGCTATGATGCACAGTTCGAAGTTATTAAAGGCCAGACCATTGAAGTTCGCTGTGAAAGTATTAGCGGCACCCTGCCGATTAGTTATCAGCTGCTGAAAACCAGTAAAGTGCTGGAAAATAGCACCAAAAATAGCAATGATCCGGCAGTGTTCAAAGATAATCCGACCGAAGATGTGGAATATCAGTGCGTTGCAGATAATTGTCATAGCCATGCCAAAATGCTGAGCGAAGTTCTGCGTGTTAAAGTGATTGCACCGGTGGATGAAGTGCAGATTAGCATTCTGAGCAGCAAAGTGGTGGAAAGCGGTGAAGATATTGTGCTGCAGTGTGCCGTTAATGAAGGCAGTGGTCCGATTACCTATAAATTCTATCGTGAAAAAGAGGGTAAGCCGTTCTATCAGATGACCAGTAATGCCACCCAGGCCTTCTGGACCAAACAGAAAGCAAGTAAAGAACAGGAAGGTGAATATTATTGTACCGCCTTCAATCGCGCCAATCATGCAAGCAGTGTTCCGCGTAGTAAAATTCTGACCGTTCGTGTGATTCTGGCCCCGTGGAAAAAA

Restriction Sites: NdeI-XhoI

Background: CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~63kDa

Notes: For research use only, not for use in diagnostic procedure.