Product Description

CD317 Polyclonal Antibody

Catalogue Numbers: NCP0356P-50, NCP0356P-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: Q10589

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB: 1:5000~1:10000

Background: BST2 (CD317, Tetherin, HM1.24) is a type II transmembrane glycoprotein functioning as a major mediator of the innate immune defense against the dissemination of enveloped viruses by tethering virion on the cell surface. BST2 has an N-terminal cytoplasmic tail for endocytosis and cytoskeletal signaling, a transmembrane domain, an extracellular domain containing putative disulfide bonds and coiled coil region for forming homodimer, and a C-terminal GPI domain for membrane anchoring. Both the transmembrane domain and the GPI domain can insert either to the cell membrane or the viral envelope membrane and hold them together to prevent viral release. Some viruses encode proteins, such as HIV-1 and Vpu respectively, to act as antagonists to counteract BST2. BST2 is overexpressed in gastrointestinal cancers, breast cancer, lung cancer, and multiple myeloma. BST2 monoclonal antibody targeting myeloma or lung cancer cells induces cellular cytotoxicity and cell death (ADCC, antibody-dependent cell-mediated cytotoxicity). Thus, BST2 serves as a potential target for tumor immunotherapy.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD317 polyclonal antibody detects endogenous levels of CD317 protein.

Extra Notes: Western blot (WB) analysis of CD317 polyclonal antibody at 1:5000 dilution
Lane1:The Kidney tissue lysate of Mouse(30ug)
Lane2:L02 cell membrane lysate(24ug)

Bioworld Molecular Weight: ~ 30/60 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Bone marrow stromal antigen 2; BST-2; HM1.24 antigen; Tetherin; CD317; BST2

Immunogen: Recombinant protein of human CD317.

Conjugate: Unconjugated

Modification: Unmodified