Product Description

CD321/JAM-A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90233-50, BS90233-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y624(Human) O88792(Mouse) Q9JHY1(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:1,000-1:5,000
IHC:1:50-1:200

Background: Junctional adhesion molecule (JAM) is a member of the immunoglobulin superfamily expressed in tight junctions of epithelial cells and endothelial cells. It is implicated in transendothelial migration of leukocytes. JAM is constitutively expressed on circulating monocytes, neutrophils, lymphocyte subsets and platelets. The JAM family consists of JAM-A, JAM-B and JAM-C, alternatively designated JAM-1, JAM-2 and JAM-3, respectively. JAM-A localizes with F-Actin at the cell-cell contacts and at the membrane ruffles. It is involved in cell to cell adhesion through homophilic interactions and plays a role in the organization of tight junctions and modulation of leukocyte extravasation. JAM-B interacts with discrete subsets of PBLs, suggesting that it may play a role in lymphocyte trafficking. JAM-B and JAM-C proteins are binding partners; JAM-C may be a functional JAM-B receptor. Specifically, JAM-B adheres to T cells through heterotypic interactions with JAM-C. The JAM-B/JAM-C interaction my play a role in T, NK and dendritic cellular inflammation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD321/JAM-A polyclonal antibody detects endogenous levels of CD321/JAM-A protein.

Bioworld Molecular Weight: 33 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of JAM-1 on different lysates using anti-JAM-1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Human liver, Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-JAM-1 antibody. Counter stained with hematoxylin.

Alternative Name: JAM-A, JAM-1, PAM-1, F11R, CD 321, CD321, CD321 antigen, ESTM33, F11 receptor, F11R, JAM 1, JAM A, JAM, JAM-1, JAM-A, JAM1, JAM1, JAMA, JCAM, Jcam1, Junction adhesion molecule 1, Junction adhesion molecule, mouse, homolog of, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, Ly106, PAM 1, PAM-1, PAM1, Platelet adhesion molecule 1, Platelet adhesion molecule, Platelet F11 receptor, PRO301, UNQ264,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified