Product Description

CD338 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0298-500, NCP0298-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: ABCG2 (BCRP1/ABCP/MXR) is a member of the ATP-binding cassette transporter family that functions as ATP-dependent transporters for a wide variety of chemical compounds and are associated with drug-resistance in cancer cells. ABCG2 is a heavily glycosylated transmembrane protein with six transmembrane spanning regions consistent with it functioning as a half-transporter. The ABC family can exist as either fμll-length transporters or as half-transporters that form functional transporters through homo- or heterodimerization. High expression of ABCG2 was found in placenta as well as cell lines selected for resistance to a number of chemotherapeutic drugs, including mitoxantrone, doxorubicin, topotecan and flavopiridol. In rodents, the highest expression of ABCG2 was found in kidney. ABCG2 expression has also been observed in stem cell popμlations, particμlarly in hematopoietic and neuronal stem cells and is downregμlated with differentiation.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~43kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: Q9UNQ0

Tag: His-tag

Amino Acid Sequence: AGCAGTAGCAATGTGGAAGTGTTCATTCCGGTGAGCCAGGGTAATACCAATGGCTTCCCGGCAACCGCAAGCAATGATCTGAAAGCCTTCACCGAAGGCGCAGTGCTGAGCTTCCATAATATCTGTTATCGCGTTAAACTGAAAAGTGGCTTCCTGCCGTGTCGTAAACCGGTGGAAAAAGAAATTCTGAGTAATATTAACGGCATCATGAAACCGGGCCTGAATGCAATTCTGGGTCCGACCGGCGGCGGTAAAAGTAGCCTGCTGGATGTGCTGGCCGCACGCAAAGATCCGAGTGGTCTGAGCGGTGATGTGCTGATTAATGGTGCCCCGCGCCCGGCAAACTTCAAATGCAATAGCGGTTATGTGGTGCAGGATGATGTGGTTATGGGTACCCTGACCGTGCGTGAAAATCTGCAGTTCAGTGCCGCCCTGCGTCTGGCAACCACCATGACCAATCATGAAAAAAATGAACGTATCAACCGCGTTATTCAGGAACTGGGTCTGGATAAAGTTGCCGATAGCAAAGTTGGCACCCAGTTCATTCGCGGTGTTAGCGGTGGCGAACGCAAACGTACCAGTATTGGCATGGAACTGATTACCGATCCGAGCATTCTGTTCCTGGATGAACCGACCACCGGCCTGGATAGCAGTACCGCAAATGCAGTGCTGCTGCTGCTGAAACGTATGAGCAAACAGGGTCGTACCATTATCTTCAGCATTCATCAGCCGCGTTATAGTATCTTCAAACTGTTCGATAGTCTGACCCTGCTGGCAAGTGGTCGCCTGATGTTCCATGGTCCGGCCCAGGAAGCACTGGGTTACTTCGAAAGCGCAGGCTATCATTGTGAAGCCTATAATAATCCGGCCGACTTCTTCCTGGATATTATTAATGGTGATAGTACCGCAGTGGCCCTGAATCGCGAAGAAGACTTCAAAGCCACCGAAATTATTGAACCGAGTAAACAGGATAAACCGCTGATTGAAAAACTGGCCGAAATCTATGTTAATAGTAGCTTCTATAAGGAGACCAAAGCCGAACTGCATCAGCTGAGCGGTGGTGAAAAAAAAAAAAAGATTACCGTGTTCAAGGAGATTAGTTATACCACCAGCTTCTGCCATCAGCTGCGCTGGGTTAGCAAACGCAGCTTCAAAAATCTGCTGGGCAATCCGCAGGCCAGC

Expression Vector: pet-22b(+)

Research Use Only