Product Description

CD34 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90234-50, BS90234-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P28906

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:1,000-1:2,000
ICC:1:200
IHC:1:2,000-1:3,000
FC:1:50-1:100

Background: CD34 is a heavily glycosylated type I transmembrane molecule, that can be phoshorylated by a variety of kinases including Protein kinase C and Tyrosine kinases. CD34 antigen is expressed on small vessel endothelial cells and tumors of epithelial origin. CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. So CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD34 polyclonal antibody detects endogenous levels of CD34 protein.

Bioworld Molecular Weight: 120 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD34 on different cell lysates using anti-CD34antibody at 1/1000 dilution.
Positive control:
Lane 1: Human brain
Lane 2: Mouse brain
Lane 3: Mouse testis
Lane 4: Mouse thymus
Lane 5: Mouse spleen
Lane 6: Jurkat
Lane 7: TF-1
Lane 8: F9, ICC staining CD34 in hES cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CD34, CD34, CD34 antigen, CD34 molecule, CD34, Cluster designation 34, Hematopoietic progenitor cell antigen CD34, HPCA1, Mucosialin, OTTHUMP00000034733, OTTHUMP00000034734,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified