Product Description

CD34 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0202-500, NCP0202-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CD34 is a type I transmembrane glycophosphoprotein expressed by hematopoietic stem/progenitor cells, vascμlar endothelium and some fibroblasts . CD34 expression has been the hallmark used to identify hematopoietic stem cells for many years. CD34+ hematopoietic stem cells expand and differentiate into all the lymphohematopoietic lineages upon cytokine or growth factor stimμlation and lose CD34 expression upon differentiation. However, recent studies performed in various laboratories conflict with that convention. The extracellμlar domain of CD34 is homologous to CD43, a protein involved in cell-cell adhesion, and CD34 has been shown to function as a negative regμlator of cell adhesion. CD34 associates with CrkL but not CrkII, is a substrate for PKC, and activation of PKC is coupled with surface expression of CD34.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~34kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P28906

Tag: His-tag

Amino Acid Sequence: AGTCTGGATAATAACGGCACCGCAACCCCGGAACTGCCGACCCAGGGCACCTTCAGCAATGTTAGCACCAATGTTAGCTATCAGGAAACCACCACCCCGAGCACCCTGGGCAGCACCAGCCTGCATCCGGTTAGCCAGCATGGTAATGAAGCAACCACCAATATTACCGAAACCACCGTTAAATTCACCAGCACCAGTGTTATTACCAGCGTGTATGGTAATACCAATAGCAGTGTGCAGAGTCAGACCAGCGTTATTAGTACCGTGTTCACCACCCCGGCAAATGTTAGCACACCGGAAACCACCCTGAAACCGAGTCTGAGTCCGGGCAATGTGAGTGATCTGAGTACCACCAGTACCAGTCTGGCAACCAGCCCGACCAAACCGTATACCAGTAGCAGCCCGATTCTGAGTGATATTAAAGCCGAAATTAAGTGTAGCGGCATTCGTGAAGTTAAACTGACCCAGGGTATCTGTCTGGAACAGAATAAAACCAGCAGCTGCGCCGAATTCAAAAAAGATCGCGGTGAAGGTCTGGCCCGCGTGCTGTGCGGCGAAGAACAGGCAGATGCAGATGCAGGTGCCCAGGTGTGTAGCCTGCTGCTGGCACAGAGTGAAGTTCGTCCGCAGTGCCTGCTGCTGGTGCTGGCAAATCGTACCGAAATTAGTAGCAAACTGCAGCTGATGAAAAAACATCAGAGCGATCTGAAAAAACTGGGCATTCTGGACTTCACCGAACAGGATGTGGCAAGTCATCAGAGTTATAGCCAGAAAACC

Expression Vector: pet-22b(+)

Research Use Only