Product Description

CD366 Monoclonal Antibody

Catalogue Numbers:MB65850-50, MB65850-100

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: Q8TDQ0

Host: Mouse

Reactivity: Human

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype. Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced anti-tumor responses in a mouse model of colorectal cancer. In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease. Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death.

Purification and Purity: The monoclonal antibody was affinity-purified from Mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bioworld Molecular Weight: ~ 33 kDa

Specificity: Recognizes human CD366

Note: For research use only, not for use in diagnostic procedure.
Alternative Name: TIM3; TIMD3; Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3

Immunogen: Human Tim-3 fusion protein

Conjugate: Unconjugated

Modification: Unmodified