Product Description

CD3EAP (E474) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3852-50, BS3852-100

Sizes: 50µl, 100µl

Swiss-Prot: O15446

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

Background: CD3EAP, also known as ASE1, CAST or PAF49, is a 510 amino acid protein that localizes to the nucleus and belongs to the eukaryotic RPA34 RNA polymerase subunit family. Existing as two alternatively spliced isoforms, CD3EAP functions as a component of the Pol I complex, specifically exhibiting DNA-dependent RNA polymerase activity and effectively catalyzing the the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. Isoform 2 of CD3EAP may be a component of the T cell receptor (TCR) complex and is subject to tyrosine phosphorylation in response to TCR stimulation. Both isoforms of CD3EAP are subject to DNA damage-dependent phosphorylation, probably by ATM or ATR.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CD3EAP (E474) polyclonal antibody detects endogenous levels of CD3EAP protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of CD3EAP (E474) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 55, 75 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49; ASE1; CAST; PAF49

Immunogen: Synthetic peptide, corresponding to amino acids 451-500 of Human CD3EAP.

Conjugate: Unconjugated

Modification: Unmodified