Product Description

CD3EAP Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA23052-50, RA23052-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling., function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation., function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex., miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function., PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes., PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Belongs to the eukaryotic RPA34 RNA polymerase subunit family., subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes., subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.,

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, IHC-p, IF (paraffin section), ELISA

Alternate Names: CD3EAP, ASE1, CAST, PAF49, DNA-directed RNA polymerase I subunit RPA34, A34.5, Antisense to ERCC-1 protein, ASE-1, CD3-epsilon-associated protein, CAST, CD3E-associated protein, RNA polymerase I-associated factor PAF49

Clonality: Polyclonal

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Molecular Weight: 55

Protein Molecular Weight (kDa): 55

Storage: -20°C/1 year

Human Gene ID: 10849

Human SwissProt Number: O15446

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only