Product Description

CD3Z  Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: NCP0206P-50, NCP0206P-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellμlar signal transduction machinery. This activation process depends mainly on CD3 (Cluster of Differentiation 3), a mμltiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecμles such as ZAP-70 and the p85 subunit of PI-3 kinase. TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck. The CD3ζ invariant chain is a type-I transmembrane protein that exists in the TCR signaling complex as a disμlfide-linked homodimer. The cytoplasmic tail of each CD3ζ monomer contains three distinct ITAM motifs, each containing two tyrosine residues. Phosphorylation of CD3ζ ITAM tyrosine residues, including Y142, is driven by recruitment of the Lck and Fyn tyrosine kinases to the TCR. Lck/Fyn-mediated ITAM phosphorylation creates docking sites that promote the SH2 domain-dependent recruitment and activation of Zap-70, which drives amplification of signaling events downstream of the TCR that facilitate T cell activation. Phosphorylation of a pool of p16 CD3ζ leads to the generation of p21 and p23 species, which differ in the degree of ITAM phosphorylation. It has been proposed that the ratio of p21/p23 contributes to regμlating the amplitude of T cell activation. CD3ζ plays an important role in the assembly and surface expression of the TCR complex. Indeed, research studies have demonstrated that CD3ζ is degraded in response to Ag-dependent TCR stimμlation as a mechanism to tightly control T cell activation.

Category: Primary Antibody

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: IF

Alternate Names: T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Recombinant protein of human CD3Z.

Dilution: IF: 1:100~1:500

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CD3Z polyclonal antibody detects endogenous levels of CD3Z protein.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P20963

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only