Product Description

CD4 Recombinant Protein

Catalogue Number: NCP0207-500, NCP0207-1000

Sizes: 500ug, 1mg

Swiss-Prot: P01730

Host: E.coli

Tag: His-tag

AA Sequence: AAGAAGGTGGTTCTGGGCAAAAAAGGTGATACCGTGGAACTGACCTGTACCGCAAGTCAGAAAAAAAGTATTCAGTTCCATTGGAAAAACAGCAATCAGATTAAAATCCTGGGTAATCAGGGTAGCTTCCTGACCAAAGGTCCGAGCAAACTGAATGATCGCGCCGATAGCCGTCGTAGTCTGTGGGATCAGGGTAACTTCCCGCTGATTATTAAAAATCTGAAAATCGAAGACAGCGATACCTATATCTGTGAAGTGGAAGATCAGAAAGAAGAAGTGCAGCTGCTGGTGTTCGGTCTGACCGCCAATAGTGATACCCATCTGCTGCAGGGTCAGAGCCTGACCCTGACCCTGGAAAGCCCGCCGGGTAGCAGCCCGAGTGTTCAGTGTCGTAGTCCGCGCGGCAAAAATATTCAGGGTGGCAAAACCTTAAGTGTTAGCCAGCTGGAACTGCAGGATAGCGGCACCTGGACCTGTACCGTTCTGCAGAATCAGAAAAAAGTTGAATTCAAAATCGACATCGTGGTTCTGGCATTCCAGAAAGCCAGCAGCATTGTGTATAAAAAAGAAGGCGAACAGGTGGAATTCAGCTTCCCGCTGGCCTTCACCGTGGAAAAACTGACCGGCAGCGGTGAACTGTGGTGGCAGGCCGAACGCGCCAGCAGCAGTAAAAGTTGGATTACCTTCGATCTGAAAAATAAAGAAGTGAGCGTTAAACGTGTTACCCAGGATCCGAAACTGCAGATGGGTAAAAAACTGCCGCTGCATCTGACCCTGCCGCAGGCACTGCCGCAGTATGCCGGCAGTGGTAATCTGACCCTGGCCCTGGAAGCCAAAACCGGCAAACTGCATCAGGAAGTTAATCTGGTTGTGATGCGTGCAACCCAGCTGCAGAAAAATCTGACCTGCGAAGTGTGGGGCCCGACCAGCCCGAAACTGATGCTGAGCCTGAAACTGGAAAATAAAGAAGCCAAAGTGAGTAAACGTGAAAAAGCAGTGTGGGTTCTGAATCCGGAAGCAGGCATGTGGCAGTGTCTGCTGAGCGATAGCGGTCAGGTTCTGCTGGAAAGCAATATTAAAGTTCTGCCGACCTGGAGCACCCCGGTTCAGCCG

Restriction Sites: NdeI-XhoI

Background: Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation.

Soluble: PBS, 4M Urea, PH7.4

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Expression Vector: pet-22b(+)

BioWorld Molecular Weight: ~45kDa

Notes: For research use only, not for use in diagnostic procedure.