CD40 Ligand Polyclonal Antibody
Catalogue Numbers: BS60466-50, BS60466-100
Sizes: 50µl, 100µl
Alternative Name: CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40LG; CD40L; TNFSF5; TRAP
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P29965
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: CD40 Ligand polyclonal antibody detects endogenous levels of CD40 Ligand protein.
Bioworld Molecular Weight: ~ 29, 35 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of CD40 Ligand polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Rat spleen tissue lysate
Immunogen: A synthetic peptide corresponding to residues in Human CD40 Ligand.
Conjugate: Unconjugated
Modification: Unmodified