CD40L Polyclonal Antibody-BS90246

CD40L Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90246-50, BS90246-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29965(Human) P27548(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. While cytokines IL-4 and IL-13 alone are inadequate for B cell activation, contact with Th cells seems to be sufficient for delivery of proliferative signals. CD40 and CD154 (also designated CD40L or TRAP) comprise a receptor ligand pair central to the transmission of this signal. CD40 is expressed on the surface of B cells and CD154 is expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. CD154 is a 261 amino acid protein that is is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. Its expression is tightly regulated, and abnormal levels of CD154 are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD40L polyclonal antibody detects endogenous levels of CD40L protein.

Bioworld Molecular Weight: 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD40L on different cells lysates using anti-CD40L antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse liver, ICC staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CD40-L, TRAP, CD40LG, CD 40L, CD154, CD40 antigen ligand, CD40 ligand, CD40 ligand, soluble form, CD40-L, CD40L, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T B cell activating molecule, T BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TrAP, Tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified