CD44 Polyclonal Antibody-NCP0210P

CD44 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0210P-50, NCP0210P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P16070

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IF

All Applications: WB: 1:5000~1:10000
IF 1:50~1:200

Background: CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications. Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Research studies have shown that interactions between CD44 and HER2 are linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM. CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD44 polyclonal antibody detects endogenous levels of CD44 protein.

Bioworld Molecular Weight: ~ 75 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD44 polyclonal antibody at 1:5000 dilution
Lane 1: C6 whole cell lysate (30ug)
Lane 2: MCF-7 cell membrane lysate (24ug), Immunofluorescence analysis of MCF-7 cells using CD44 pAb at dilution of 1:200.

Alternative Name: CD44 antigen; CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I;
PGP-I; CD44; LHR; MDU2; MDU3; MIC4

Immunogen: Recombinant protein of human CD44.

Conjugate: Unconjugated

Modification: Unmodified