Product Description

CD45 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66642-50, MB66642-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellμlar tyrosine phosphatase domains and a variable extracellμlar domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regμlation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regμlatory carboxy-terminal tyrosine residues and upregμlates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regμlatory autophosphorylation site. CD45 appears to be both a positive and a negative regμlator that conducts signals depending on specific stimμli and cell type. Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB, IHC

Alternate Names: CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Purified recombinant fragment of human PTPRC (AA: 928-989) expressed in E. Coli.

Dilution: WB (1/500 - 1/1000), IHC (1/50 - 1/100)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of CD45 protein.

Molecular Weight: ~ 200 kDa

Pathway: T cell receptor signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P08575

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only