Product Description

CD45 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0211-500, NCP0211-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellμlar tyrosine phosphatase domains and a variable extracellμlar domain generated by alternative splicing. The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates. CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regμlation of T- and B-cell antigen receptor signaling. Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regμlatory carboxy-terminal tyrosine residues and upregμlates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regμlatory autophosphorylation site. CD45 appears to be both a positive and a negative regμlator that conducts signals depending on specific stimμli and cell type. Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~66kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P08575

Tag: His-tag

Amino Acid Sequence: CAGAGCCCTACCCCGAGCCCTACCGGTCTGACCACCGCCAAAATGCCGAGTGTTCCGCTGAGTAGCGATCCGCTGCCGACCCATACCACCGCCTTCAGCCCGGCCAGCACCTTCGAACGCGAAAATGACTTCAGCGAAACCACCACCAGCCTGAGTCCGGATAATACCAGTACCCAGGTTAGTCCGGATAGCCTGGATAATGCAAGCGCATTCAATACCACCGGTGTTAGCAGTGTGCAGACCCCGCATCTGCCGACCCACGCAGATAGCCAGACCCCGAGTGCAGGCACCGATACCCAGACCTTCAGCGGCAGCGCCGCCAATGCAAAACTGAATCCGACCCCGGGCAGTAATGCCATTAGTGATGTTCCGGGCGAACGCAGTACCGCAAGCACCTTCCCGACCGATCCGGTTAGTCCGCTGACCACCACCCTGAGTCTGGCACATCATAGTAGCGCCGCACTGCCGGCACGTACCAGCAATACCACCATTACCGCAAATACCAGTGATGCATATCTGAATGCCAGTGAAACCACCACACTGAGTCCGAGCGGTAGTGCCGTGATTAGCACCACCACCATTGCCACCACCCCGAGCAAACCGACCTGTGATGAAAAATATGCCAATATTACCGTGGATTATCTGTATAATAAGGAAACCAAACTGTTCACCGCAAAACTGAACGTGAATGAAAATGTTGAATGTGGTAATAACACCTGTACCAATAATGAAGTTCATAATCTGACCGAATGTAAAAATGCCAGCGTGAGCATTAGTCATAATAGCTGTACCGCACCGGATAAAACCTTAATTCTGGATGTTCCGCCGGGTGTGGAAAAATTCCAGCTGCATGATTGCACCCAGGTGGAAAAAGCCGATACCACCATCTGTCTGAAATGGAAAAATATTGAAACCTTCACCTGCGATACCCAGAATATTACCTATCGCTTCCAGTGCGGTAATATGATCTTCGATAATAAAGAAATCAAGCTGGAAAACCTGGAACCGGAACATGAATATAAATGTGATAGCGAAATCCTGTATAACAATCATAAATTCACCAACGCCAGTAAAATTATTAAGACCGACTTCGGCAGTCCGGGTGAACCGCAGATTATCTTCTGCCGCAGCGAAGCCGCACATCAGGGCGTGATTACCTGGAATCCGCCGCAGCGCAGCTTCCATAACTTCACCCTGTGCTATATTAAAGAAACCGAAAAAGATTGCCTGAATCTGGATAAAAATCTGATTAAATACGACCTGCAGAATCTGAAACCGTATACCAAATATGTGCTGAGTCTGCATGCATATATTATTGCCAAAGTGCAGCGCAATGGTAGTGCCGCCATGTGTCACTTCACCACCAAAAGCGCCCCGCCGAGTCAGGTGTGGAATATGACCGTGAGCATGACCAGTGATAATAGTATGCATGTGAAATGCCGCCCGCCGCGTGATCGCAATGGCCCTCATGAACGCTATCATCTGGAAGTTGAAGCAGGCAATACCCTGGTGCGCAATGAAAGCCATAAAAATTGTGACTTCCGCGTGAAAGATCTGCAGTATAGCACCGATTATACCTTCAAAGCATACTTCCATAATGGTGATTATCCGGGCGAACCGTTCATTCTGCATCATAGCACCAGTTATAATAGCAAA

Expression Vector: pet-22b(+)

Research Use Only