Product Description

CD50/ICAM-3 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0215-500, NCP0215-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cell adhesion molecμles (CAMs) are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth. These proteins are thought to play an important role in embryogenesis and development. ICAM-3, also designated CD50 and ICAM-R, is a type I membrane protein that is thought to regμlate morphological changes during cell locomotion. ICAM-3 acts as a counter-receptor for the leukocyte Integrin αL/β2, and is known to activate T cells and polymorphonuclear leukocytes. ICAM-3 also binds to Moesin, via the cytoplasmic domain of ICAM-3. The expression of ICAM-3 is induced by RANTES, a chemoattractant known to activate T lymphocytes. ICAM-3 is also a major ligand for the leukocyte Integrin LFA-1 (CD11a/CD18).

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~50kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P32942

Tag: His-tag

Amino Acid Sequence: CAGGAATTCCTGCTGCGCGTGGAACCGCAGAATCCGGTTCTGAGTGCCGGCGGTAGTCTGTTCGTTAATTGCAGTACCGATTGTCCGAGCAGTGAAAAAATTGCACTGGAAACCAGCCTGAGCAAAGAACTGGTTGCCAGCGGCATGGGTTGGGCAGCATTCAATCTGAGCAATGTGACCGGCAATAGTCGTATTCTGTGTAGCGTGTATTGTAATGGTAGTCAGATTACCGGCAGTAGCAATATTACCGTGTATCGTCTGCCGGAACGCGTGGAACTGGCCCCGCTGCCTCCGTGGCAGCCTGTTGGTCAGAACTTCACCCTGCGTTGCCAGGTGGAAGATGGCAGCCCGCGTACCAGTCTGACCGTGGTGCTGCTGCGCTGGGAAGAAGAACTGAGTCGTCAGCCGGCAGTTGAAGAACCGGCAGAAGTGACCGCCACCGTGCTGGCAAGTCGTGATGATCATGGCGCCCCGTTCAGTTGCCGTACCGAACTGGATATGCAGCCGCAGGGTCTGGGTCTGTTCGTTAACACCAGTGCACCGCGTCAGCTGCGTACCTTCGTTCTGCCGGTGACCCCGCCGCGCCTGGTTGCACCTCGCTTCCTGGAAGTTGAAACCAGTTGGCCGGTGGATTGCACCCTGGATGGCCTGTTCCCGGCCAGTGAAGCCCAGGTGTATCTGGCACTGGGTGATCAGATGCTGAATGCCACCGTTATGAATCATGGCGATACCCTGACCGCCACCGCAACCGCAACCGCGCGTGCAGATCAGGAAGGTGCACGCGAAATTGTGTGTAATGTGACCCTGGGCGGCGAACGTCGCGAAGCACGCGAAAATCTGACCGTGTTCAGCTTCCTGGGTCCGATTGTGAATCTGAGCGAACCGACCGCACATGAAGGCAGCACCGTGACCGTGAGTTGCATGGCAGGCGCACGCGTGCAGGTGACCCTGGATGGTGTGCCGGCCGCCGCACCTGGTCAGCCTGCACAACTGCAGCTGAATGCAACCGAAAGCGATGATGGTCGTAGCTTCTTCTGCAGCGCAACCCTGGAAGTTGATGGCGAATTCCTGCATCGTAATAGTAGCGTTCAGCTGCGTGTGCTGTATGGTCCGAAAATTGATCGTGCAACCTGTCCGCAGCATCTGAAATGGAAAGATAAAACCAGACATGTTCTGCAGTGTCAGGCACGTGGTAATCCGTATCCGGAACTGCGTTGTCTGAAAGAAGGTAGTAGTCGCGAAGTTCCGGTTGGTATTCCGTTCTTCGTTAATGTGACCCATAATGGCACCTATCAGTGTCAGGCCAGCAGTAGCCGCGGCAAATATACCCTGGTGGTGGTGATGGATATTGAAGCAGGCAGCAGTCAT

Expression Vector: pet-22b(+)

Research Use Only