Product Description

CD54 Monoclonal Antibody

Catalogue Numbers: MB65793-50, MB65793-100

Sizes: 50µl, 100µl

Product: Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P05362

Host: Mouse

Reactivity: Human

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: Intercellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. CD54 is expressed at low levels in diverse cell types, and is induced by cytokines (TNF-α, interleukin-1) and bacterial lipopolysaccharide. Apical localization of CD54 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier . Apical expression of CD54 on epithelial cells mediates pathogen invasion as well as host defense, a pattern also observed in tumors. CD54 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 (leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation. Cross-linking of CD54 or binding to its ligand triggers activation of Src family kinases and the Rho/ROCK pathway. Phosphorylation on Tyr485 of CD54 is required for its association with SHP-2. SHP-2 seems essential for CD54-induced Src activation.

Purification and Purity: The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes human CD54

Extra Notes: Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD54 Antibody, followed by anti-mouse IgG PE.

Bioworld Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; CD antigen CD54

Immunogen: Native purified human CD54.

Conjugate: Unconjugated

Modification: Unmodified