CD59 Polyclonal Antibody-BS90252

CD59 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90252-50, BS90252-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P13987(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF FC IP,

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
FC:1:50-1:100
IP:1:10-1:50

Background: CD59 is a GPI-anchored glycoprotein that is expressed on leukocytes, vascular endothelial cells, various epithelial cells and placenta. CD59 acts together with CD58 in mediating T cell adhesion and activation, and it may be a second ligand of CD2. CD59 functions as a regulator of the terminal pathway of complement by binding to the C8/C9 components of the assembling membrane attack complex (MAC) on host cell membranes, to stop the formation of the lytic pore. CD59 also drives both calcium release and activation of lipid-raft associated signalling molecules such as tyrosine kinases. CD59 gene has two p53-responsive domains that may be implicated in the defense of host cells from damage by the complement system in inflammation, suggesting that p53 could be used to mediate susceptibility of tumor cells to the complement lysis during chemotherapy.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD59 polyclonal antibody detects endogenous levels of CD59 protein.

Bioworld Molecular Weight: 14/20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution.
Positive control:
Lane 1: Human placenta
Lane 2: HUVEC
Lane 3: K562, ICC staining CD59 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: HRF-20, HRF20, MAC-IP, MACIF, MIRL, CD59, 16.3A5, 1F5, 1F5 antigen, 20 kDa homologous restriction factor, CD 59, CD_antigen=CD59, CD59, CD59 antigen, CD59 antigen complement regulatory protein, CD59 antigen p18 20, CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344), CD59 glycoprotein, CD59 molecule, CD59 molecule complement regulatory protein, CD59, Cd59a, Complement regulatory protein, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF 20, HRF-20, HRF20, Human leukocyte antigen MIC11, Ly 6 like protein, Lymphocytic antigen CD59/MEM43, MAC inhibitory protein, MAC IP, MAC-inhibitory protein, MAC-IP, MACIF, MACIP, MEM43, MEM43 antigen, Membrane attack complex (MAC) inhibition factor, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MGC2354, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Protectin, Surface antigen recognized by monoclonal, 16.3A5, T cell activating protein,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified