Product Description

CD63 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0219P-50, NCP0219P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P08962

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IF

All Applications: WB: 1:1000~1:2000
IF 1:50~1:200

Background: CD63 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin-enriched microdomains (TEMs) where they mediate a range of processes, including adhesion, motility, membrane organization, and signal transduction. CD63, like other tetraspanins, is enriched in exosomes. It is also a component of Weibel-Palade bodies found in endothelial cells. Research studies demonstrate several functions of CD63 in different cell types, including roles in mast cell degranulation, VEGF signaling in endothelial cells, recruitment of leukocytes to endothelial cells, and endosomal sorting during melanogenesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD63 polyclonal antibody detects endogenous levels of CD63 protein.

Bioworld Molecular Weight: ~ 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD63 polyclonal antibody at 1:1000 dilution
Lane 1: EC9706 cell membrane lysate (12ug)
Lane 2: The Liver tissue lysate of Mouse (24ug)
Lane 3: The Heart tissue lysate of Rat (24ug), Immunofluorescence analysis of SGC7901 cells using CD63 pAb at dilution of 1:200.

Alternative Name: CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Lysosome integral membrane protein 1; Limp1; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD63; CD63; MLA1; TSPAN30

Immunogen: Recombinant protein of human CD63.

Conjugate: Unconjugated

Modification: Unmodified