Product Description

CD66/CEACAM1 Recombinant Protein

Sizes: 500μg, 1mg

Catalogue Numbers: NCP0220-500, NCP0220-1000

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: CEACAM1 (also known as C-CAM and CD66a) is a member of CEA-related cell-adhesion molecμle (CEACAM) subfamily of the carcinoembryonic antigen (CEA) family. CEACAM1 is expressed by certain epithelial, endothelial, lymphoid, and myeloid cells. Human CEACAM1 has many different splice variants; the abundance of CEACAM1 and the relative ratio of the different isoforms varies markedly among cell types and may be regμlated in a context-dependent fashion. The isoforms with long (L) and short (S) cytoplasmic tails have different signaling properties. Notably, L isoforms contain a functional ITIM (immunoreceptor tyrosine-based inhibitory motif) and several serine and threonine residues that coμld serve as potential phosphorylation targets. The extracellμlar domain of CEACAM1 is heavily glycosylated, making its apparent molecμlar weight during electrophoresis much larger than its predicted size (57.6 kDa). CEACAM1 mediates intercellμlar adhesion through homo- and heterophilic interaction with other members of the CEACAM family. Studies indicate that CEACAM1 plays important roles in angiogenesis, neovascμlarization, insμlin signaling, T cell signaling, and tumorigenesis. In addition, CEACAM1 can function as a receptor for several microbial pathogens.

Category: Protein

Host: E. coli

Purification and Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Molecular Weight: ~45kDa

Solubility: PBS, 4M Urea, PH7.4

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restriction Sites: NdeI-XhoI

SwissProt: P13688

Tag: His-tag

Amino Acid Sequence: CAGCTGACCACCGAAAGCATGCCGTTCAATGTGGCCGAAGGCAAAGAAGTTCTGCTGCTGGTTCATAATCTGCCGCAGCAGCTGTTCGGTTATAGTTGGTATAAAGGTGAACGCGTGGATGGTAATCGCCAGATTGTGGGTTATGCCATTGGTACCCAGCAGGCAACCCCGGGTCCGGCTAATAGTGGCCGCGAAACCATCTATCCGAATGCCAGCCTGCTGATTCAGAATGTTACCCAGAATGATACCGGCTTCTATACCCTGCAGGTGATTAAAAGTGATCTGGTTAATGAAGAAGCCACCGGTCAGTTCCATGTGTATCCGGAACTGCCGAAACCGAGCATTAGTAGCAATAATAGTAATCCGGTGGAAGATAAAGATGCAGTTGCCTTCACCTGTGAACCGGAAACACAAGATACCACCTATCTGTGGTGGATTAATAATCAGAGTCTGCCGGTTAGCCCGCGCCTGCAGCTGAGCAATGGTAATCGCACCCTGACCCTGCTGAGCGTTACCCGTAATGATACCGGTCCGTATGAATGCGAAATTCAGAATCCGGTTAGCGCAAATCGCAGCGATCCGGTTACCCTGAATGTTACCTATGGCCCGGATACCCCGACCATTAGTCCGAGCGATACCTATTATCGTCCGGGTGCAAATCTGAGTCTGAGCTGCTATGCAGCAAGCAATCCGCCGGCACAGTATAGCTGGCTGATTAATGGCACCTTCCAGCAGAGTACCCAGGAACTGTTCATTCCGAATATTACCGTTAATAATAGCGGTAGCTATACCTGTCATGCAAATAATAGTGTTACCGGTTGCAATCGTACCACCGTTAAAACCATTATTGTGACCGAACTGAGCCCGGTGGTTGCCAAACCGCAGATTAAAGCAAGTAAAACCACCGTTACCGGCGATAAAGATAGCGTTAATCTGACCTGTAGTACCAATGATACCGGAATTAGCATTCGTTGGTTCTTCAAAAATCAGAGCCTGCCGAGTAGCGAACGCATGAAACTGAGCCAGGGCAATACCACCCTGAGTATTAATCCGGTGAAACGTGAAGATGCCGGCACCTATTGGTGCGAAGTGTTCAATCCGATTAGTAAAAATCAGTCAGATCCGATTATGCTGAATGTTAATTATAACGCCCTGCCGCAGGAAAATGGTCTGAGCCCGGGC

Expression Vector: pet-22b(+)

Research Use Only