Product Description

CD67 Polyclonal Antibody

Catalogue Numbers: BS60329-50, BS60329-100

Sizes: 50µl, 100µl

Alternative Name: Carcinoembryonic antigen-related cell adhesion molecule 8; CD67 antigen; Carcinoembryonic antigen CGM6; Non-specific cross-reacting antigen NCA-95; CD66b; CEACAM8; CGM6

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P31997

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Carcinoembryonic antigen-related cell adhesion molecule 8, also designated CD67, CD66b or nonspecific cross-reacting antigen (NCA-95), belongs to the human carcinoembryonic antigen (CEA) family. The CD67 antigen is encoded by the CEACAM8 (CGM6) gene, which is exclusively expressed in neutrophils and eosinophils. In neutrophils, the CEACAM8 gene is primarily detected in the secondary granules within the cytoplasm, but it can also be found in lower amounts on the plasma membrane. The amount of CD67 on the plasma membrane is up-regulated upon granulocyte activation. CD67 has been located on the surface of neutrophilic and eosinophilic granulocytes at late stages of differentiation. It exhibits heterophilic cell adhesion properties with CD66c, which is coexpressed with CD67 in granulocytes. CD67, which is attached to the membrane by a GPI-anchor, is expressed in leukocytes of chronic myeloid leukemia patients and bone marrow and in granulocytes in the spleen, thymus and lungs.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD67 polyclonal antibody detects endogenous levels of CD67 protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD67 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: NIH-3T3 cell lysate
Lane 3: PC12 cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human CD67.

Conjugate: Unconjugated

Modification: Unmodified