Product Description

CD74 Monoclonal Antibody

Catalogue Numbers: MB66794-50, MB66794-100

Sizes: 50µl, 100µl

Product: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P04233

Host: Mouse

Reactivity: Human

Applications: WB, FC

All Applications: WB (1/500 - 1/1000), FC (1/10 - 1/50)

Background: The human histocompatibility leukocyte antigen (HLA) class II-associated invariant chain is composed of at least four polypeptides. One of these polypeptide chains is expressed as a membrane-bound subunit and has been designated CD74. The loading of peptide onto the class II MHC protein (MHC II) appears to be regulated by CD74, which associates with MHC II during its migration to the endosomal compartment, where class II binds peptide. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. In fact, CD74 is broadly expressed in normal B lymphocytes, regardless of their histocompatibility leukocyte antigen (HLA) phenotype, while a subset of peripheral T lymphocytes that are MHC II negative do not express CD74.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of CD74 protein.

Extra Notes: Western blot analysis of CD74 expression in Daudi (A), Raji (B) whole cell lysates.

Bioworld Molecular Weight: ~ 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DHLAG; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD74

Immunogen: KLH-conjugated synthetic peptide of human CD74. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified