CD83 Polyclonal Antibody-BS67542

CD83 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67542-50, BS67542-100

Sizes: 50µl, 100µl

Swiss-Prot: Q01151

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: CD83 is a single-transmembrane protein with a calculated molecular weight (MW) of 23 kDa, but due to heavy and differential glycosylation, its apparent MW ranges from 23 to 70 kDa. CD83 is predominantly expressed on mature dendritic cells (DCs) and has been used as a DC activation/maturation marker as its increased expression is correlated with upregulation of HLA class II antigen expression on DCs . CD83 is also expressed at a low level on lymphocytes and is upregulated upon lymphocyte activation . Thymic epithelial cells also express CD83, which is required for normal CD4+ T cell development. CD83 is also expressed as a soluble form (sCD83) that can be found in serum of healthy adults. sCD83 has been shown to negatively regulate immune response by lymphocytes .

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of CD83 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD83 expression in rat thymus (A), mouse lung (B), HuT78 (C) whole cell lysates., Immunohistochemical analysis of CD83 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 44 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: CD83 antigen; hCD83; B-cell activation protein; Cell surface protein HB15; CD83

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD83. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified