CD86 Polyclonal Antibody-BS65646

CD86 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65646-50, BS65646-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O35531

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC IP,

All Applications: WB: 1:100-1:500
IF/ICC: 1:50-1:200
IP: 1:50-1:200

Background: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011].

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD86 Polyclonal Antibody detects endogenous levels of CD86 protein.

Bioworld Molecular Weight: ~ 65-70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CD86 antibody at 1:500 dilution., Immunofluorescence analysis of THP-1 cells using CD86 Polyclonal Antibody at dilution of 1:100 (40x lens)., Immunofluorescence analysis of rat spleen using CD86 Polyclonal Antibody at dilution of 1:100 (40x lens)., Immunoprecipitation analysis of 200 μg extracts of Jurkat cells using 3 μg CD86 antibody. Western blot was performed from the immunoprecipitate using CD86 antibody at a dilution of 1:1000.

Alternative Name: CD28LG2; FUN1; LAB72; Activation B7 2 antigen; Activation B7-2 antigen 3; CD86_HUMAN; Activation B7-2 antigen; Activation B72 antigen; B lymphocyte activation antigen B7 2; B lymphocyte activation antigen B72; B-lymphocyte activation antigen B7-2 2; B-lymphocyte activation antigen B7-2; B7 2; B7 2 antigen; B7; B7-2; B7.2; B70; B72 antigen; BU63; CD 86; CD28 antigen ligand 2 2; CD28 antigen ligand 2; Cd28l2; CD28LG2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2; CD86 antigen; CD86 molecule; CLS1; CTLA 4 counter receptor B7 2; CTLA 4 counter receptor B7.2; CTLA-4 counter-receptor B7.2 2, 3; CTLA4 counter receptor B72; Early T-cell costimulatory molecule 1; ETC-1; FUN 1; LAB72; Ly-58; Ly58; MB7; MB7-2; Membrane glycoprotein; MGC34413; T lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; TS/A-2

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-247 of human CD86 (NP_787058.4).

Conjugate: Unconjugated

Modification: Unmodified