CD86 Rat Monoclonal Antibody-MB65837

CD86 Rat Monoclonal Antibody

Catalogue Numbers:MB65837-50, MB65837-100

Product: Rat IgG2a kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P42082

Host: Rat

Reactivity: Mouse

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation. CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions. Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity.

Purification and Purity: The monoclonal antibody was affinity-purified from rat antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bioworld Molecular Weight: ~ 34 kDa

Specificity: Recognizes mouse CD86

Note: For research use only, not for use in diagnostic procedure.
Alternative Name: CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86

Immunogen: LPS-activated CBA/Ca mouse splenic B cells

Conjugate: Unconjugated

Modification: Unmodified