Product Description

CD8a Rat Monoclonal Antibody

Catalogue Numbers:MB65828-50, MB65828-100

Product: Rat IgG2a kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.

Swiss-Prot: P01731

Host: Rat

Reactivity: Mouse

Applications: IF, FC

All Applications: IF (1/50 - 1/200)

Background: Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation.

Purification and Purity: The monoclonal antibody was affinity-purified from rat antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bioworld Molecular Weight: ~ 27 kDa

Specificity: Recognizes mouse CD8a

Note: For research use only, not for use in diagnostic procedure.
Alternative Name: MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a

Immunogen: Mouse thymus or spleen

Conjugate: Unconjugated

Modification: Unmodified