CD8B Polyclonal Antibody-BS60358-B

CD8B Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS60358-B-50, BS60358-B-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P10966

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:1000~1:2000
IHC: 1:50~1:100

Background: The T cell receptor (TCR) is a heterodimer composed of either α and β or γ and δ chains. CD3 chains and the CD4 or CD8 co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8. T helper cells express CD4 proteins and T cytotoxic cells display CD8. CD8 (also designated Leu 2 or T8), a cell surface glycoprotein, is a two chain complex (αα or αβ) receptor that binds class I MHC molecules presented by the antigen-presenting cell (APC). A primary function of CD8 is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. An additional role for CD8-expressing T cells may be to maintain low levels of HIV expression.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD8B polyclonal antibody detects endogenous levels of CD8B protein.

Bioworld Molecular Weight: ~ 30kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD8B polyclonal antibody at 1:1000 dilution
Lane 1: The spleen tissue lysate of Mouse (40ug)
Lane 2: Myla2059 whole cell lysate (40ug), Immunohistochemistry of paraffin-embedded Human stomach cancer using CD8B antibody at dilution of 1:50 (40x lens).

Alternative Name: T-cell surface glycoprotein CD8 beta chain; CD8b; CD8B

Immunogen: Synthetic peptide, corresponding to Human CD8B.

Conjugate: Unconjugated

Modification: Unmodified