Product Description

CD97 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: NCP0237P-50, NCP0237P-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P48960

Host: Rabbit

Reactivity: Rat, Mouse,

Applications: WB,

All Applications: WB: 1:2000~1:5000

Background: CD97 is a member of the EGF-TM7 (seven-span transmembrane) protein family, which is characterized by an extended extracellular region with a variable number of N-terminal EGF-like domains coupled to a TM7 stalk. It is expressed by leukocytes following activation. CD97 binds to its cellular ligand CD55 (decay accelerating factor) and protects several cell types from complement-mediated damage. The CD97-CD55 interaction may play a role in cellular activation, migration and adhesion following inflammation. CD97 expression is increased in thyroid cancer, paralleling dedifferentiation and tumor staging in this disease. Many colorectal cell lines are also CD97+, with CD97 levels correlating with migration and invasion in vitro. CD97 is also expressed in various gastric, pancreatic and esophageal carcinomas. CD97 shares significant homology with EMR2, however the two proteins exhibit different expression patterns, as EMR2 is not expressed in any of the aforementioned cancer cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CD97 polyclonal antibody detects endogenous levels of CD97 protein.

Bioworld Molecular Weight: ~ 97 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CD97 polyclonal antibody at 1:2000 dilution
Lane 1: The Heart tissue lysate of Mouse (30ug)
Lane 2: The Heart tissue lysate of Rat (30ug)

Alternative Name: Adhesion G protein-coupled receptor E5; Leukocyte antigen CD97; CD97; Adhesion G protein-coupled receptor E5 subunit alpha; Adhesion G protein-coupled receptor E5 subunit beta; ADGRE5; CD97

Immunogen: Recombinant protein of human CD97.

Conjugate: Unconjugated

Modification: Unmodified