Product Description

Cdc25C (phospho-S216) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4035-50, BS4035-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cyclin dependent kinases are positively regμlated by association with cyclins and negatively regμlated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regμlated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Cdc25C around the phosphorylation site of Serine 216.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-CDC25C (S216) polyclonal antibody detects endogenous levels of CDC25C protein only when phosphorylated at Ser216.

Molecular Weight: ~ 60 kDa

Pathway: G Protein-coupled Receptors Signaling to MAPK Erk, Cell Cycle G2 M DNA Damage Signaling Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P30307

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only