Cdc27 (K390) Polyclonal Antibody
Catalogue Numbers: BS2200-50, BS2200-100
Sizes: 50µl, 100µl
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P30260
Host: Rabbit
Reactivity: Human
Applications: WB, IHC
All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Cdc27 (K390) polyclonal antibody detects endogenous levels of Cdc27 protein
Extra Notes: Western blot (WB) analysis of Cdc27 (K390) pAb at 1:500 dilution
Lane1:MCF-7 whole cell lysate(40ug)
Lane2:HEK293T whole cell lysate(40ug)
Lane3:K562 whole cell lysate(40ug)
Bioworld Molecular Weight: ~ 80, 92 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Name: Cell division cycle protein 27 homolog; Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC; CDC27; ANAPC3; D0S1430E; D17S978E
Immunogen: Synthetic peptide, corresponding to amino acids 360-410 of Human Cdc27.
Conjugate: Unconjugated
Modification: Unmodified