Product Description

Cdc34 (D163) Polyclonal Antibody

Catalogue Numbers: BS2949-50, BS2949-100

Sizes: 50µl, 100µl

Alternative Name: Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1; UBCH3; UBE2R1; CDC34

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49427

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1/S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cdc34 (D163) polyclonal antibody detects endogenous levels of Cdc34 protein.

Bioworld Molecular Weight: ~ 26 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cdc34 (D163) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate(40ug)
Lane 2: HepG2 whole cell lysate(40ug)
Lane 3: The Lung tissue lysate of Mouse(40ug)
Lane 4: PC12 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 150-200 of Human Cdc34.

Conjugate: Unconjugated

Modification: Unmodified