Product Description

Cdc34 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90270-50, BS90270-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49427(Human) Q8CFI2(Mouse) Unigene:2427(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IP, , FC

All Applications: WB:1:1,000-1:5,000
FC:1:50-1:100
ICC:1:100-1:500

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cdc34 polyclonal antibody detects endogenous levels of Cdc34 protein.

Bioworld Molecular Weight: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cdc34 on different lysates using anti-Cdc34 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: Jurkat, ICC staining Cdc34 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CDC34, Cdc 34, Cdc34, Cell division cycle 34, Cell division cycle 34 homolog (S. cerevisiae), Cell division cycle 34 homolog, E2 CDC34, UB2R1, UBC 3, UBC3, UBCH3, UBE2 R1, UBE2R1, Ubiquitin carrier protein, Ubiquitin conjugating enzyme Cdc34, Ubiquitin conjugating enzyme E2 32 kDa complementing, Ubiquitin protein ligase, Ubiquitin protein ligase R1, Ubiquitin-conjugating enzyme E2 R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified