Cdc37 (H9) Polyclonal Antibody-BS1735

Cdc37 (H9) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1735-50, BS1735-100

Sizes: 50µl, 100µl

Swiss-Prot: Q16543

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitinconjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphasepromoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation..

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cdc37 (H9) polyclonal antibody detects endogenous levels of Cdc37 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cdc37 (H9) polyclonal antibody at 1:500 dilution Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: Hela whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: MEF whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Cdc37 (H9) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 44 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Hsp90 co-chaperone Cdc37; Hsp90 chaperone protein kinase-targeting subunit; p50Cdc37; CDC37; CDC37A

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human Cdc37.

Conjugate: Unconjugated

Modification: Unmodified