CDC40 Polyclonal Antibody-BS90272

CDC40 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90272-50, BS90272-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60508(Human) Q9DC48(Mouse) EntrezGene:361859(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc40, also known as pre-mRNA-processing factor 17 (PRPF17) or EH-binding protein 3 (EHB3), is a 579 amino acid nuclear protein. Cdc40 is essential for the catalytic step II of the pre-mRNA splicing process, in which Cdc40 associates with the spliceosome C complex. Cdc40 contains seven WD repeats, which are important in protein-protein interactions. Cdc40 has sequence similarity to the yeast protein Prp17, which is involved in pre-mRNA splicing and cell cycle progression. The sequence similarity between the mammalian Cdc40 and the yeast Prp17 may indicate an additional role in cell cycle progression for mammalian Cdc40.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CDC40 polyclonal antibody detects endogenous levels of CDC40 protein.

Bioworld Molecular Weight: 66 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CDC40 on Hela cells lysates using anti-CDC40 antibody at 1/2,000 dilution., ICC staining CDC40 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Ehb3, hPRP17, Cdc40, Cell division cycle 40, Cell division cycle 40 homolog, EH binding protein 3, EH-binding protein 3, Ehb3, hPRP17, Pre mRNA processing factor 17, Pre mRNA splicing factor 17, Pre-mRNA-processing factor 17, PRP17, PRP17 homolog, PRP17, PRPF17,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified