Product Description

Cdc40 (S209) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3374-50, BS3374-100

Sizes: 50µl, 100µl

Swiss-Prot: O60508

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Cdc40, also known as pre-mRNA-processing factor 17 (PRPF17) or EH-binding protein 3 (EHB3), is a 579 amino acid nuclear protein. Cdc40 is essential for the catalytic step II of the pre-mRNA splicing process, in which Cdc40 associates with the spliceosome C complex. Cdc40 contains seven WD repeats, which are important in protein-protein interactions. Cdc40 has sequence similarity to the yeast protein Prp17, which is involved in pre-mRNA splicing and cell cycle progression. The sequence similarity between the mammalian Cdc40 and the yeast Prp17 may indicate an additional role in cell cycle progression for mammalian Cdc40.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Cdc40 (S209) polyclonal antibody detects endogenous levels of Cdc40 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cdc40 (S209) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (10ug)
Lane 2: MCF-7 whole cell lysate (10ug)
Lane 3: CT26 whole cell lysate (10ug)
Lane 4: The Brain tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of Cdc40 (S209) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 65 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Pre-mRNA-processing factor 17; Cell division cycle 40 homolog; EH-binding protein 3; Ehb3; PRP17 homolog; hPRP17; CDC40; EHB3; PRP17; PRPF17

Immunogen: Synthetic peptide, corresponding to amino acids 181-230 of Human Cdc40.

Conjugate: Unconjugated

Modification: Unmodified