Product Description

CDC42 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76411-50, BS76411-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P60953

Host: Rabbit

Reactivity: Human

Applications: IF/ICC

All Applications: IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Immunogen: Recombinant fusion protein of human CDC42(NP_001782.1).

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunofluorescence analysis of U2OS cells using CDC42 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.