Product Description

CDC45 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76608-50, BS76608-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75419

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Immunofluorescence analysis of A-549 cells using cdc45 antibody . Blue: DAPI for nuclear staining.

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: CDC45; CDC45L; CDC45L2; MGORS7; PORC-PI-1; cdc45

Immunogen: Recombinant fusion protein of human CDC45(O75419).

Conjugate: Unconjugated

Modification: Unmodified