CDC45 Polyclonal Antibody-BS90275

CDC45 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90275-50, BS90275-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75419(Human) Q9Z1X9(Mouse) EntrezGene:287961(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 and Cdc45 are the mammalian homologs of S. cerevisiae Cdc6 and Cdc45, which are involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of S. cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CDC45 polyclonal antibody detects endogenous levels of CDC45 protein.

Bioworld Molecular Weight: 66 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining CDC45 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining CDC45 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CDC45, CDC 45, CDC 45L, CDC45 (cell division cycle 45, S.cerevisiae homolog) like, CDC45, CDC45 cell division cycle 45 like, CDC45 like, CDC45 related protein, CDC45, CDC45L2, Cell division control protein 45 homolog, Cell division cycle 45 like 2, Cell division cycle 45 like, PORC PI 1, PORC-PI-1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified